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ykoH [2019-06-07 10:11:50]
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ykoH [2019-06-07 10:11:50]

two-component sensor kinase
locus
BSU13260
pI
6.17
mw
50.49 kDa
protein length
454 aa Sequence Blast
gene length
1365 bp Sequence Blast
function
unknown
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    1,392,643 1,394,007

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG]

    • Paralogous protein(s)

  • [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|KinE], [protein|4EE48E4931F662E51586DB6D01E91C688329222D|CssS], [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG]

    • [SW|Domains]

  • two transmembrane segments, C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|5C93] (Lactobacillus plantarum [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK], corresponds to aa 230 ... 441, 32% identity) [pubmed|28994408]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|6A37531896205A9B894C81AB0563C216C0B52CD7|ykoG]-[gene|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|ykoH]-[gene|0678357183072D2716B913C07103CEBF65E60D14|ykoI]-[gene|348EAFC260435B3E0ED3CC7BC27EBA8DCFCBBEDC|ykoJ]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B312 (ykoH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1311 NBRP B. subtilis, Japan]
  • BKE13260 ([gene|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|ykoH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCTTGGTCTTCAGCTTCA, downstream forward: _UP4_AGCGAACAGAATGGGGGAGG
  • BKK13260 ([gene|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|ykoH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCTTGGTCTTCAGCTTCA, downstream forward: _UP4_AGCGAACAGAATGGGGGAGG

    • References

  • 10094672,28994408